Page 438 - Demo

442 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYThe results of Table 2 show that the optimal pH for the hydrolysis of cellulose in sugarcane bagasse combined with surfactants is between 4.5 and 5.0. Samples with Tween 20, 80 and Triton - X 100 were compared to the control sample and the results show insignificant improvement, when adding surfactants, the glucose content produced fluctuated from 148.6g /L (control sample) to 154.5 g/L (Triton - X 100). However, at pH 5, the glucose content was significantly improved, increasing from 146.2 g/L to 157.5 g/L, while increasing the hydrolysis efficiency of cellulose to a maximum of 70%. This may be because most cellulase enzymes have an isoelectric point (pI) of about 4.8 and when pH increases, pI exceeding 4.8 will reduce the catalysis of cellulase to cellulose fibers in sugarcane bagasse. According to the study of Lou et al. [10], the addition of surfactants can change the surface charge of cellulose substrate and cellulase enzyme. This also shows that pH affects the improvement of the impacts of non-ionic surfactants to support the enzyme to participate in hydrolysis catalysis to achieve higher efficiency.Effect of pHShaking speed is also an important factor affecting the catalysis of enzymes with substrates and enzymes with surfactants. The properties of sugars produced during hydrolysis of cellulose increase the viscosity of the hydrolyzed solution. If hydrolysis is carried out for a long time, the viscosity will be higher, affecting the catalysis of enzymes and surfactants with the cellulose of bagasse. The survey was conducted at shaking speeds of 100 rpm, 150 rpm, and 200 rpm with 5g/L of Tween 20, Tween 80, and Triton-X 100, respectively. The results are shown in Table 3.