Page 437 - Demo

SECTION II. APPLIED BIOTECHNOLOGY IN THE PHARMACEUTICAL INDUSTRY... 441However, when the solid residue concentration increases, the hydrolysis efficiency decreases, the decrease is nearly 50%, the glucose content is only 74.3 g/L, compared to the solid residue concentration at 5%, the glucose content is 140.5 g/L. At a high solid residue concentration, high viscosity causes difficulty in mixing the residue, which is also a factor that hinders the hydrolysis process and reduces the hydrolysis efficiency. According to some studies, bagasse can be hydrolyzed at high solid residue concentration, however, hydrolysis at high solid residue concentration leads to a water deficit, affecting the hydrolysis rate. The water deficit, high viscosity and low mixing ability are all challenges for this process [9]. Therefore, in the hydrolysis process at high solid residue concentration, a big enough mixing device must be used to quickly liquefy the residue to increase glucose content in the hydrolyzed solution.Effect of pHpH is also considered an important factor in the enzymatic hydrolysis process using surfactants, pH plays an important role in promoting the conversion of cellulose into sugar in the hydrolysis of sugarcane bagasse cellulose. Based on the optimal pH range of the active enzyme and the subsequent fermentation process, experiments were conducted at pH ranges of 4.0; 4.5; 5.0; 5.5 and 6.0. The results are shown in Table 3.2.Table 2. Effect of pH on the efficiency of hydrolysis of cellulose in sugarcane bagasseSurfactantEfficiency of hydrolysis of cellulose in sugarcane bagasse (%)4.0 4.5 5.0 5.5 6.0Control 40.2 %u00b1 0.40 66.1 %u00b1 0.38 65.0 %u00b1 0.39 58.7 %u00b1 0.08 39.6 %u00b1 0.08Tween 20 43.3 %u00b1 0.29 68.5 %u00b1 0.25 68.2 %u00b1 0.35 59.2 %u00b1 0.31 45.3 %u00b1 0.12Tween 80 39.9 %u00b1 0.64 67.6 %u00b1 0.75 69.8 %u00b1 0.60 58.5 %u00b1 0.25 39.9 %u00b1 0.17Triton X-100 40.4 %u00b1 0.14 68.7 %u00b1 0.10 70.0 %u00b1 0.21 60.1 %u00b1 0.15 40.0 %u00b1 0.25