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438 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYIn which: Cglucose: Glucose content in hydrolyzed solution (g/L)V : Volume of experimental sample (L)1.1 : Conversion factor of glucan to glucose A : Amount of residue glucan used (%)m : amount of analyzed sample calculated on absolute dry weight (g) 3. RESULTS3.1. Effect of surfactants on the hydrolysis of cellulose in sugarcane bagasseThe purpose of the fermentation process to produce ethanol is to obtain a large enough sugar content during the enzymatic hydrolysis of cellulose, however, the use of enzymes is related to the selection of the type of enzymes to achieve high efficiency, low-cost, hydrolysis ability at high solid residue concentration, etc. The research on the hydrolysis of cellulose in sugarcane bagasse aims to determine the hydrolysis conditions to recover high sugar solution for the fermentation process to produce ethanol.Tween and Triton X-100 are non-ionic surfactants, containing hydrophilic heads and hydrophobic tails. Surfactants interact with lignin through hydrophobic interactions and can increase the efficiency of the activity of cellulase enzyme in the hydrolysis of lignocellulosic biomass from sugarcane bagasse pretreated with formic acid. The effect of surfactants Tween 20, Tween 80 and Triton X-100 on glucose concentration and hydrolysis efficiency of cellulose in sugarcane bagasse were studied. The results are shown in Figure 1.