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RESEARCH ON THE EFFECT OF SURFACTANTS ON THE HYDROLYSIS OF LIGNOCELLULOSE IN SUGARCANE BAGASSEMai Nguyen Thi Phuong1,*, Nam Nguyen Van2, Minh Doan Tuan1Abstract: Non-ionic surfactants are commonly known to improve the enzymatic hydrolysis of lignocellulose from sugarcane bagasse. However, studies have shown that the combination of surfactants with Cellic Ctec 2 enzyme at optimal hydrolysis conditions also needs to be further studied to evaluate the efficiency of hydrolysis of cellulose along with the amount of glucose released. In this research, the combination of Triton X %u2013 100 with Cellic Ctec 2 enzyme at a concentration of 5 FPU/mL under the conditions of 5% solid concentration, pH 5, shaking speed of 150 rpm significantly improved the amount of glucose released in the hydrolysate. Under these conditions, surfactants can enhance the conversion of cellulose, affecting the adsorption process of celulase enzyme, leading to the amount of glucose released after 60 hours of hydrolysis reaching 180 g/L, increasing the hydrolysis efficiency to 80.4%. In addition, Triton X %u2013 100 was 1 Faculty of Environment, Hanoi University of Natural Resources and Environment; ntpmai@hunre.edu.vn2 Faculty of Infrastructural Engineering and Urban Environment, Hanoi Architectural University* Correspondence: ntpmai@hunre.edu.vn