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SECTION II. APPLIED BIOTECHNOLOGY IN THE PHARMACEUTICAL INDUSTRY... 435hydrolysate such as: hydrophobicity, hydrogen bonding capacity and surface area. Another research by Zhang et al. [6] also showed that the use of surfactants improves the enzymatic hydrolysis of sugarcane bagasse. All lignocellulose compounds from sugarcane bagasse pretreated with metal salts gave a maximum glucose recovery efficiency of 88% compared to biomass treated with AlCl3. This result also helps reduce ineffective adsorption due to the interaction between Tween 80 and lignin to provide more cellulase for hydrolysis. The use of surfactants improves the efficiency of enzymatic hydrolysis of lignocellulosic materials, however, further research is still needed to select surfactants with ideal concentrations to increase the highest glucose recovery efficiency for the purpose of fermentation to produce bioethanol in the future. 2. MATERIALS AND METHODS 2.1. MaterialsSugarcane bagasse: Collected from Lam Son Sugar Factory - Thanh Hoa, dried at 700C for 24 hours and ground to 1mm size. The chemical composition of bagasse was analyzed by NREL method [7]. The sample was hydrolyzed at 300C in H2SO4 72% for 60 minutes. After hydrolysis at 300C, the sample was transferred to a conical flask 250ml and 84ml of deionized water was added, sterilized at 1210C for 60 minutes. The chemical composition of bagasse includes: Cellulose 43.5%; hemicellulose 20.5%; acidsoluble lignin 4.95%; acid-insoluble lignin 21.75%; ash 0.87% and other components 8.43%. Surfactants used in the research: Tween 20, Tween 80, Triton X-100 (mL/g) were purchased from Sigma-Aldrich, USA and enzyme Cellic Ctec 2 with activity of 238 FPU/mL was from Novozymes, Denmark.