Page 435 - Demo

SECTION II. APPLIED BIOTECHNOLOGY IN THE PHARMACEUTICAL INDUSTRY... 439Figure 1. Effect of surfactants on glucose concentration and hydrolysis efficiency of cellulose in hydrolyzed solution of sugarcane bagasse The results in Figure 1 show that the addition of surfactants improved the hydrolysis process. The change of Triton X - 100 was different after one hour of hydrolysis, the accumulated glucose concentration reached 35 g/l and the hydrolysis efficiency of cellulose reached 15.6% (increasing 2.5 times) compared to the control sample without surfactants, as well as the samples with surfactants Tween 20 and 80. However, at the following hydrolysis durations, the amount of sugar produced decreased, after 48 hours of hydrolysis Tween 80 gave the highest hydrolysis efficiency of cellulose with 100.2 g/l glucose produced and the hydrolysis efficiency reached 42.3%. When surfactant was added, a significant improvement was observed in 2 hours of hydrolysis, however, the hydrolysis reaction rate increased insignificantly and decreased gradually at the end of the period, indicating that inhibitors were present that affected the hydrolysis efficiency. According to the study of Zhou Y et al., Tween 20 and 80 had good ability of hydrolysis of lignocellulose pretreated with dilute acid at a solid concentration of about 5 g/L [8]. The study also showed that the inhibition of non-ionic surfactants on the hydrolysis of pure cellulose may be due to the interaction between the surfactants and the cellulase enzyme, leading to