Page 432 - Demo

436 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGY2.2. Research methodsPretreatment and enzymatic hydrolysisThe pretreatment was carried out in stainless steel high pressure reactors with a capacity of 500ml. The reaction was carried out with about 5% dry weight of sugarcane bagasse with formic acid solution 80% at a solid/liquid ratio of 1:10 (w/v) at 1300C for 50 minutes. After fractionation, the sample was separated by filtration on filter paper (Whatman No.4) and washed with alkali 0.5% at temperature 600C for 30 minutes. The bagasse after being washed with alkali was washed again with deionized water until pH 7 was reached. The solid obtained after pretreatment was stored at 40C for further use.The pretreated sugarcane bagasse was hydrolyzed with Cellic Ctec 2 enzyme with an activity of 238 FPU/mL. Enzymatic hydrolysis was carried out in sodium acetate buffer 0.05M at pH 4.8 with a total hydrolysis volume of 10ml, the experiment was carried out with a bagasse concentration of 5% (w/v). The control flasks were determined without surfactant addition and enzyme concentration of 10 FPU/mL, the experimental samples were carried out at a surfactant concentration of 5 g/L and enzyme activity of 5 FPU/mL. The mixture was stirred on a thermostatic shaker at 500C with a shaking speed of 150 rpm for 72 hours. The experiments were carried out in triplicate to obtain the average value to determine the glucose concentration through hydrolysis conditions with solid residue concentration varying from 5 - 20%, pH 4.0 - 6.0, shaking speed from 100 - 200 rpm.Determination of glucose contenta. Determination of glucose content by D-Glucose GOD-PODThe sample after being hydrolyzed had the enzyme inactivation at 950C for 5 minutes, then cooled to room temperature. The sample was shaken to mix well and diluted to a