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440 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYinhibition of hydrolysis of cellulose. Many studies have shown that the addition of surfactants at appropriate concentrations can improve the hydrolysis of cellulose and the interaction between surfactants and enzyme Cellic Ctec 2 depends on several factors such as surfactant type, concentration, pH, solids ratio, etc. Therefore, it is necessary to further research the effects of factors with surfactants on the efficiency of hydrolysis of cellulose in sugarcane bagasse.3.2. Effect of hydrolysis conditions of cellulose in sugarcane bagasseEffect of solid residue concentrationTo evaluate the relationship between the solid residue concentration and surfactant on the efficiency of enzymatic hydrolysis of cellulose, the experiment was conducted to hydrolyze cellulose in sugarcane bagasse at solid residue concentrations varying from 5%, 10%, 15%, 20% (w/v), enzyme activity 5 FTU/mL, at pH 4.8, shaking speed 150 rpm for 72 hours. The results are shown in Table 1.Table 1. Effect of the solid residue concentration on the efficiency of hydrolysis of cellulose in sugarcane bagasse SurfactantEfficiency of hydrolysis of cellulose in sugarcane bagasse (%)5% 10% 15% 20%Control 52.5 %u00b1 0.47 47.3 %u00b1 0.54 35.3 %u00b1 0.15 31.3 %u00b1 0.56Tween 20 43.8 %u00b1 0.45 37.1 %u00b1 0.45 30.4 %u00b1 0.52 25.9 %u00b1 0.38Tween 80 48.2 %u00b1 0.10 35.1 %u00b1 0.16 34.6 %u00b1 0.15 30.1 %u00b1 0.21Triton X-100 62.5 %u00b1 0.47 51.7 %u00b1 0.40 37.5 %u00b1 0.45 33.0 %u00b1 0.55The results in Table 1 show that the hydrolysis efficiency of cellulose in bagasse changes significantly when the solid residue concentration increases under the simultaneous effect of both Tween 20, 80 and Triton X-100. For Triton X-100, at a solid residue concentration of 5%, the hydrolysis efficiency of cellulose reaches the highest at 62.5% (about 1.4 times higher than Tween 20 and 80).