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434 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYgathered to provide rigidity and strength to plants and resist the decomposition of bacteria and the impacts of enzyme.Lignocellulosic biomass from sugarcane bagasse, rice husk, leaves, straw, corn cobs, hardwood, softwood, etc. are all abundant sources of raw materials for biofuel production. However, lignocellulosic biomass has a complex structure and is not easily decomposed by microorganisms or enzymes [3]. To break down the stable structure of lignocellulose in biomass, it is necessary to combine physical, chemical and biological factors for pretreatment before proceeding with bioethanol fermentation, which is also an important factor in fractional hydrolysis of lignocellulosic biomass. In addition, there are still difficulties in the process of enzymatic hydrolysis of lignocellulose such as: consuming a large amount of enzyme, low hydrolysis efficiency, low concentration of solids used in hydrolysis, etc. The use of additives in the hydrolysis process shows that it can solve the above disadvantages. In industry, surfactants play an important role in biofuel production plants in particular and many other fields in general, molecules such as surfactants can be used to increase the saccharification yield of lignocellulosic biomass [3].In this research, hydrolysis using surfactants was associated with changes in the structure, such as solubilization of lignin components or an increase in the surface area of cellulose in the hydrolysate[4]. Surfactants can be added during enzymatic hydrolysis, mainly non-ionic surfactants. According to the research of Chen et al. [5], it was demonstrated that the addition of Tween 20 improved the efficiency of enzymatic hydrolysis of wheat straw, increasing the conversion efficiency of cellulose to glucose by up to 80%. The research also showed that Tween 20 not only disrupted the interaction between lignin - cellulase bonds but also changed the properties of lignin in the