Page 442 - Demo

446 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGY34.5 g/L; 55.7 g/L in the experimental samples supplemented with Tween 20, Tween 80 and Triton X-100; corresponding to hydrolysis efficiencies of 12.4%; 15.3% and 24.8%. After 60 hours of hydrolysis, the efficiency increased to 68.5%; 78.3%; 75.9% and 80.4%, respectively. According to the study of Kim et al. [12, 13], surfactants also increase the reaction area by swelling the fibers, enhancing the combination of surfactants with lignin and preventing ineffective adsorption between enzymes and lignin. During 24 hours of hydrolysis, the glucose concentration accumulated in the experiments with added surfactants was equal to that of the control sample at 36 hours of hydrolysis, which was 102.2 g/L. This showed that the addition of surfactants could reduce hydrolysis time while releasing the same amount of glucose. However, when the glucose concentration increased for a long time, the hydrolysis efficiency increased slowly. This also showed that the released glucose increased the viscosity of the hydrolyzed solution and was also an inhibitor leading to a decrease in hydrolysis efficiency. According to the study of Andri%u0107 P et al. [14], the amount of glucose produced in the hydrolyzed solution ranging from 0.1 - 319 g/L reduces the hydrolysis reaction rate. The results of the study showed that the addition of surfactants could reduce the amount of enzymes by half, leading to a significant reduction in the cost of enzymatic hydrolysis.4. DISCUSSION4.1. Mechanism of non-ionic surfactants Non-ionic surfactants have the ability to reduce the surface tension and change the structure of plant biomass, and can be used as additives in the enzymatic hydrolysis of plant biomass to improve the efficiency of hydrolysis. According to some studies,