Page 161 - Demo

SECTION I: MOLECULAR GENETIC ENGINEERING AND BIOCHEMICAL TECHNOLOGY 165Table 2. The paw edema inhibition at different times on Formalin-induced paw edema modelGroup Paw edema inhibition (%)0.5 hour 1 hour 2 hours 3 hours 5 hours 24 hours1 (negative control) - - - - - -2 (NanofibersEPMC 15% 1.0 mg)4.72 %u00b1 0.078.61 %u00b1 0.01 15.23 %u00b1 0.0512.21 %u00b1 0.06 13.79 %u00b1 0.0612.50 %u00b1 0.063 (nanofibersEPMC 15% 2.0 mg)9.45 %u00b1 0.3218.54 %u00b1 0.17 24.37 %u00b1 0.0922.09 %u00b1 0.06 17.24 %u00b1 0.0817.61 %u00b1 0.054 (Free EPMC 1.0 mg) 3.94 %u00b1 0.197.28 %u00b1 0.11 14.72 %u00b1 0.1011.63 %u00b1 0.06 14.94 %u00b1 0.0711.93 %u00b1 0.065 (free EPMC 2.0 mg) 7.09 %u00b1 0.2517.22 %u00b1 0.22 23.35 %u00b1 0.0320.93 %u00b1 0.15 17.82 %u00b1 0.0314.77 %u00b1 0.046 (positive control) 5.51 %u00b1 0.1025.17 %u00b1 0.41 25.89 %u00b1 0.0633.72 %u00b1 0.15 38.51 %u00b1 0.1345.45 %u00b1 0.193.2. Anti-inflammatory activity on carrageenan-induced paw edema modelIn carrageenan-induced paw edema model assay, the oral anti-inflammatory effect of nanofibers-EPMC 15% and free EPMC was evaluated by comparing paw edema percentage of treatment groups and vehicle group at 3, 5, 6, and 7 hours after carrageenan injection. The results (Table 3) revealed that nanofibers-EPMC 15% and free EPMC at a dose of 200mg/kgP showed acute antiinflammatory effect at 5, 6 and 7 hours after carrageenan injection (p <0.05). Their effect was weaker than that of indomethacin 10 mg/kgP at 5 hours after carrageenan injection (p<0.05) but equivalent to that of indomethacin 10 mg/kgP at 6 and 7 hours after carrageenan injection (p>0.05). At a dose of 100 mg/kgP, both nanofibers-EPMC 15% and free EPMC did not significantly inhibited paw edema in mice at the studied time points.