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SECTION I: MOLECULAR GENETIC ENGINEERING AND BIOCHEMICAL TECHNOLOGY 163(8 mice per each group, n=8) including group 1 (vehicle group, oral administration of CMC 0.1% at a dose of 1ml/ 100g body weight), group 2 (positive control group, indomethacin 10 mg/kg body weight), groups 3, 4, 5, 6 (treatment groups, nanofibersEPMC 15% at a dose of 100 and 200 mg/kg body weight, free EPMC at a dose of 100 and 200 mg/kg body weight, respectively). The paw edema was induced by injecting carrageenan 1% into the plantar side of right hind paw 30 minutes after treatment with sample. The paw volume of the mice was measured at 3, 5, 6, and 7 hours after carrageenan injection. Paw edema percentage was calculated using formula (1):Where Vt was the paw volume at time t after carrageenan injection, Vo was the paw volume before carrageenan injection.2.4. Statistical analysisThe data were processed by SPSS 16.0 software. The results were presented as mean %u00b1 SD (standard deviation). One-way Anova test and post-hoc test were used for statistical comparison between different groups. The differences with P<0.05 were considered statistically significant.3. RESULTS3.1. Anti-inflammatory activity on formalin-induced paw edema modelThe results of topical anti-inflammatory activity evaluation on formalin-induced mouse paw edema model are shown in Table 1 and 2. The paw thickness of experimental mice at different times is shown in Table 1. The results revealed that nanofibers-EPMC 15% and free EPMC at a dose of 1.0 mg produced insignificantly