Page 158 - Demo

162 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYlaboratory animals in this study were conducted according to ethical guidelines and approval from the Scientific Council of the Institute of Biotechnology, VAST for animal studies.2.2. Evaluation of anti-inflammatory activity using formalin-induced paw edema modelNanofibers-EPMC 15% (electrospun nanofiber loaded with 15% ethyl-p-methoxycinnamate) and free EPMC (100% ethyl-p-methoxycinnamate) were powdered then dissolved in DMSO at different concentrations (1.0 mg/40 %u00b5L DMSO and 2.0 mg/40 %u00b5L DMSO). The in vivo anti-inflammatory assay was done according to the method described by Pashmforosh et al [10]. 48 mice were divided into six groups (8 mice per each group, n=8). Group 1 and 2 was respectively applied 40 %u00b5L of the solution per paw at the contents of 1.0 mg and 2.0 mg nanofibers-EPMC 15%. Group 3 and 4 was respectively applied 40 %u00b5L of the solution per paw at the contents of 1.0 mg and 2.0 mg free EPMC. Group 5 applied with 2.0 mg of diclofenac diethylamine emulgel per paw was used as the drug control. Group 6 applied 40 %u00b5L DMSO was used as the negative control. Thirty minutes later, the paw edema was induced by injecting 20 %u00b5L of 10% formalin into the dorsal hind paw of mice. The paw thickness was measured at 0.5, 1, 2, 3, 5 and 24 hours after formalin injection using a Mitutoyo caliper.2.3. Evaluation of anti-inflammatory activity using carrageenan-induced paw edema modelThe in vivo anti-inflammatory assay using carrageenaninduced paw edema model was done according to the method described by Mansouri et al [11] with some modification to suit the laboratory conditions. 48 mice were divided into six groups