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SECTION I: MOLECULAR GENETIC ENGINEERING AND BIOCHEMICAL TECHNOLOGY 161Ethyl-p methoxycinnamate (EPMC), a natural potential antiinflammatory and analgesic agent isolated from Kaemferia galangaL., the family Zingiberaceae [5]. Nowadays, encapsulation of active ingredients in polymeric materials has gained significant attention of researchers in order to enhance their stability and bioavailability as well as achieve controlled release [6]. Electrospinning method has been considered an effective, flexible encapsulation method with high loading capacity or encapsulation efficiency due to its high porosity, large specific surface area [7, 8]. We previously reported the fabrication of the nanofibers composed of three polymer including chitosan (CS), polylactic acid (PLA), and polyethylene glycol (PEG) loaded with 15% EPMC using electrospinning method in an accepted paper for publication [9]. In the current paper, we report the results of evaluating the in vivo anti-inflammatory activity of electrospun nanofibers CS/PEG/PLA loaded with 15% EPMC using carrageenan and formalin-induced mouse paw edema models.2. MATERIALS AND METHODS 2.1. MaterialsThe electrospun nanofibers CS/PEG/PLA loaded with 15% EPMC were supplied by Department of Pharmaceutical Chemistry and Organic Synthesis the Institute of Natural Products Chemistry, VAST, Vietnam. Healthy white mice, weighing about 21-24 grams, regardless of breed, were raised at the Institute of Biotechnology VAST, Vietnam under standard conditions of temperature and light. Formalin solution, DMSO, diclofenac diethylamine emulgel, carrageenan, indomethacin (Merck, US). The care and use of