Page 163 - Demo

SECTION I: MOLECULAR GENETIC ENGINEERING AND BIOCHEMICAL TECHNOLOGY 167mechanisms of action [12-14]. Paw edema, induced in mice by administration of formalin or carrageenan, gives a close resemblance to human arthritis. In conditions like rheumatoid arthritis or osteoarthritis, inflammation occurs in the joints, leading to swelling, pain, and stiffness. The paw edema model is often used in research to study the inflammatory processes associated with arthritis and to evaluate the efficacy of antiinflammatory treatments [15, 16].In this study, the oral and topical anti-inflammatory activity of nanofibers loaded with 15% EPMC was investigated. At the same time, we also evaluated the activity of free EPMC to compare the activity of nanofibers-EPMC 15% with that of free EPMC. The results showed that both exhibited dose-dependent antiinflammatory activity on the formalin and carrageenan-induced mouse paw edema model. In the topical anti-inflammatory assay in the formalin-induced paw edema model, nanofibers-EPMC 15% and free EPMC at a dose of 2.0 mg inhibited paw edema at 2 and 3 hours after formalin injection. The activity at 2 hours was better than that at 3 hours after formalin injection. At 2 hours after formalin injection, the mouse paw edema inhibitory ability of nanofibers-EPMC 15% and free EPMC was 24.37 and 23.35% respectively, which was almost equivalent to the positive control diclofenac diethylamine emulgel at a dose of 2.0 mg with inhibition of 25.89%. In the oral anti-inflammatory assay in carrageenan-induced paw edema model, the anti-inflammatory effect of nanofibersEPMC 15% and free EPMC was evaluated by comparing paw edema percentage of treatment groups and vehicle group at 3, 5, 6, and 7 hours after carrageenan injection. The results (Table 3) revealed that nanofibers-EPMC 15% and free EPMC at a dose of 200mg/kgP showed acute anti-inflammatory effects at 5, 6 and 7 hours after injection (p<0.05). Their effect was weaker than that of