Page 113 - Demo
P. 113
SECTION I: MOLECULAR GENETIC ENGINEERING AND BIOCHEMICAL TECHNOLOGY 117260%u00b0C, while the detector temperature was maintained at 240%u00b0C. The GC temperature program started at 60%u00b0C for 2 minutes, then increased at 4%u00b0C/min up to 220%u00b0C, where it was held for an additional 10 minutes.Component Identification: Components were identified based on Retention Indices (RI), determined under the same chromatographic conditions using n-alkanes (C7-C30) as standards. The results were further validated by comparing mass spectra with the NIST 14 and Wiley 08 libraries, as outlined by Adam (2017). The component percentages in the YBA sample were calculated based on chromatographic peak areas, with no correction factors applied.3. RESULTS3.1. Oil extractionIn this study, 2 kg of camphor wood was processed by shaving and grinding to achieve a particle size of 1 Mesh. The prepared wood sample was then subjected to steam distillation for 6 hours, following the extraction procedure outlined in Figure 1. The distillation process yielded essential oil fractions, which collected in a funnel containing the condensate. In this setup, the condensate separated into three distinct layers including light oil (LO), denser oil (HO), and water layer (as shown in Figure 3). The top layer, a lighter-than-water essential oil, yielded 6 mL (LO), while the bottom layer, a heavier-than-water essential oil, yielded 0.75 mL (HO).