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116 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGY2.4.2. CytotoxicityMTT Assay: After treatment, cells were incubated with 10 %u00b5L of MTT reagent (5 mg/mL) for 4 hours.Formazan Solubilization: Following incubation, the medium was removed, and the resulting formazan crystals were dissolved in 100 %u00b5L of DMSO. Optical density was measured at 540 nm. Wells containing only culture medium served as the negative control, while wells with 100% cell growth served as the positive control.Data Calculation: Cell viability (%) was calculated using the formula:Evaluation of NO Inhibition: Test sample concentrations with >80% cell viability were further evaluated for % NO inhibition.2.5. Gas Chromatography-Mass SpectrometryPreparation of Sample for Gas Chromatography: The dried sample (the dehydrated essential oil of C. camphora) was dissolved in 1 mL of pure hexane at a 1% concentration, using Na2SO4 as a drying agent.Gas Chromatography-Mass Spectrometry (GC-MS) Analysis: The chemical composition of the YBA samples was qualitatively and quantitatively determined using Gas Chromatography coupled with Mass Spectrometry (GC-MS) on a THERMO SCIENTIFIC Trace 1310 system paired with an ITQ 900 ion trap detector (Thermo). The analysis was performed using a TG-5MS analytical column (30 m %u00d7 0.25 %u03bcm %u00d7 0.25 mm), with helium as the carrier gas. The injector temperature was set at