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114 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYexperiment repeated in triplicate (n = 3) [8]. A calibration curve (Figure 2) is constructed to illustrate the correlation between DPPH concentration and optical density:Figure 2. Calibration curve of DPPH assay2.4. Anti-inflammatory activity2.4.1. Nitric Oxide (NO) inhibition activityCell Line: RAW 264.7 cells (ATCC%u00ae-TIB-71%u2122) cultured in Dulbecco%u2019s Modified Eagle%u2019s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic solution. The cells were maintained at 37%u00b0C with 5% CO2 [9].Reagents and Chemicals: Dulbecco%u2019s Modified Eagle%u2019s Medium (DMEM), fetal bovine serum (FBS), AntibioticAntimycotic (Gibco), Lipopolysaccharides (LPS) from Escherichia coli, Griess reagent (Sigma), NG-Methyl-L-arginine acetate (L-NMMA) (Sigma), 3-(4,5-dimethylthiazol-2-yl)-2,5-