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SECTION I: MOLECULAR GENETIC ENGINEERING AND BIOCHEMICAL TECHNOLOGY 111distinguishing component distributions across the oil fractions and fully exploring their medicinal applications, particularly for bone and joint disorders [4].Through our study, we aim to contribute a deeper understanding of the essential oil composition by isolating and analyzing the more bioactive fraction. Using GC-MS (Gas Chromatography-Mass Spectrometry), we seek to identify the active compounds within this fraction that contribute to the overall therapeutic effects observed in traditional Red Dao medicinal practices. By isolating and characterizing these components, our work aspires to pinpoint the main active ingredients responsible for the health benefits of this traditional plant, ultimately providing insight into potential applications in modern complementary medicine. 2. MATERIALS AND METHODS 2.1. Sample collectedThe woody plants used in the study were collected from Vu Quang National Park, Ha Tinh Province, Vietnam. The specimens were identified by morphological characteristics by Mr. Bui Van Huong of the Vietnam Museum of Nature, and the specimen is also stored here.The two essential oil fractions collected by the circulating steam distillation method were evaluated for free radical scavenging activity by the DPPH method. After constructing the standard curve presented in the method section, we evaluated the test samples together with the positive control, quercertin. The results are presented in the following table:Both essential oil fractions showed the presence of DPPH free radical scavenging activity. In which, the heavier oil fraction gave