Page 116 - Demo

120 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYfractions would be essential to pinpoint the specific constituents responsible for their observed activity.3.3. Anti-inflammatory activityCamphor is well-known for its anti-inflammatory properties. Based on this, we assessed the nitric oxide (NO) production inhibitory activity of the collected essential oil fractions. Among the two fractions, the light essential oil (LO) fraction was more efficiently collected, as described in Section 3.1. The results of the anti-inflammatory evaluation are summarized in the following table.Table 2. Inhibition of nitric oxide productionNo SampleConcentration(%u00b5g/mL)Cell viability (%)NO inhibition (%)IC50(%u00b5g/mL)1 LO 100 96 7140.88 %u00b11.3 50 100 3025 100 135 100 202 HO100 82 8849.44 %u00b10,750 89 6225 98 275 100 3Positive controlL-NMMA160 85 884,08 %u00b10,540 94 7410 98 652,5 100 46Both essential oil fractions demonstrated notable antiinflammatory activity, with IC50 values of 40.99 %u00b5g/mL and 49.44 %u00b5g/mL for the LO and HO fractions, respectively. While these values indicate significant potential, they remain less potent than the positive control, L-NMMA, which achieved an IC50 of 4.08 %u00b5g/mL (Table 2).Comparatively, the activity of these fractions aligns well with other natural products. For instance, essential oil from