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SECTION I: MOLECULAR GENETIC ENGINEERING AND BIOCHEMICAL TECHNOLOGY 119Table 1. DPPH antioxidant activityNo Sample Concentration (%u00b5g/mL)Radical Scavenging (%)EC50(%u00b5g/mL)1 LO100 9137.9 %u00b13,72 50 6825 195 62 HO100 5955.8 %u00b11.63 50 2325 115 0Positive control Quercertin32 1009,8 %u00b10,30 8 462 100.5 0Both essential oil fractions from Cinnamomum camphorademonstrated notable DPPH free radical scavenging activity, indicating their potential as natural antioxidants. The IC50 value, which represents the concentration required to inhibit 50% of DPPH radicals, provides a measure of this activity.The heavier oil fraction exhibited an EC50 value of 55.8 %u00b5g/mL, reflecting moderate antioxidant activity. In contrast, the lighter oil fraction showed superior efficacy with a lower IC50 value of 37.9 %u00b5g/mL, indicating a stronger free radical scavenging potential. When compared to the positive control, quercetin, which achieved an EC50 of 9.8 %u00b5g/mL (Table 1), it is evident that the essential oil fractions are less potent. However, their activity still suggests that Cinnamomum camphora essential oils could serve as a valuable source of natural antioxidants, particularly in applications where synthetic antioxidants are not preferred.The difference in activity between the heavier and lighter fractions may be attributed to variations in their chemical composition. The lighter fraction could contain a higher concentration of active compounds with stronger antioxidant properties. Further analysis of the chemical profiles of these