Page 491 - Demo

SECTION II. APPLIED BIOTECHNOLOGY IN THE PHARMACEUTICAL INDUSTRY... 4953.4. Antioxidant activity of the crude EPS Figure 4. Antioxidant activity against DPPH (A) and hydroxyl radical (B) of the crude EPSThe in vitro antioxidant activity of the crude EPS was evaluated by measuring its DPPH and hydroxyl radical scavenging activities. It turned out that the antioxidant activity against DPPH of the EPS showed a positive correlation with its concentration (0-1200 %u00b5g/mL) (Fig. 4A). Compared to ascorbic acid, antioxidant activity of the EPS was much lower. The use of 1200 %u00b5g/mL EPS led to the highest antioxidant activity (74.4 %u00b1 2.9%) against DPPH free radicals. Similar to DPPH scavenging activity, the antioxidant activity of EPS on hydroxyl radical were in a concentration-dependent manner (Fig. 4B). The hydroxyl scavenging activity of the EPS ranged from 15.1 %u00b1 1.6% to 81.8 %u00b1 5.2% in accordance to increasing EPS concentrations. The hydroxyl scavenging activity of 1200 %u00b5g/mL EPS was 13.6% lesser than the ascorbic acid (94.8 %u00b1 3.6%).3.5. Evaluation of effect of the EPS on the shelf life of strawberryThe strawberries of both samples did not show any significant difference visually after 2 days. However, strawberres