Page 489 - Demo

SECTION II. APPLIED BIOTECHNOLOGY IN THE PHARMACEUTICAL INDUSTRY... 4933.2. Identification of EPS-producing bacterium MT12Morphological observation showed that the strain MT12 formed a white round, smooth surface, medium-sized colony on the surface of the MRS media (Fig. 2A). Using light microscopy, the strain MT12 was Gram-positive and rod-shaped. Under heat treatment, the strain did not produce spore. Sticky material around the MT12 colonies suggested the presence of EPS.The 16S rDNA gene sequence analysis revealed that the sequence information of MT12 was 99,9% similar to that of Lactiplantibacillus plantarum CIP 103151T (Accession: NR_104573), Lactiplantibacillus plantarum NRRL B-14768T (Accession: NR_042394), and Lactiplantibacillus plantarum JCM 1149T (Accession: NR_117813). A phylogenetic tree was constructed to revealed that the strain MT12 and and its closest related type strains such as L. plantarum CIP 103151T, L. plantarum NRRL B-14768T, and L. plantarum JCM 1149T were clustered into the distinct group (Fig. 2A). In combination with the morphological identification of the above colonies, the strain was identified as L. plantarum MT12. Figure 2. Colony morphology and phylogenetic tree of 16S rRNA sequences of bacterial strain MT12 and its closely related strains available on the GenBank database.