Page 490 - Demo

494 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGY3.3. Evaluation of antibacterial property of EPS MT12We investigated antibacterial activity against S. typhimurium ATCC 14028, S. aureus ATCC 6538, and S. epidermidis ATCC 12228 of EPS produced by L. plantarum MT12 and the result was shown in Fig. 3. The inhibitory effects of crude EPS (6.25, 12.5, 25, and 50 mg/mL) on 3 bacterial pathogens increased gradually with increasing concentrations of EPS. The highest antibacterial activity was recorded at 50 mg/mL EPS for S. typhimurium ATCC 14028 (19.3 %u00b1 0.9 mm) followed by S. epidermidis ATCC 12228 (12.8 %u00b1 1.1 mm), and E. coli ATCC 11105 (8.8 %u00b1 0.6 mm) (Figure 3). In contrast, no inhibition zone was observed at 6.25 mg/mL EPS. Figure 3. Antibacterial activity against S. typhimurium ATCC 14028, E. coli ATCC 11105, and S. epidermidis ATCC 12228 of EPS produced by L. plantarum MT12Further analysis revealed that the MIC values against S. typhimurium ATCC 14028, E. coli ATCC 11105, and S. epidermidisATCC 12228 of EPS from L. plantarum MT12 were 25 mg/mL, 40 mg/mL, and 35 mg/mL, respectively.