Page 383 - Demo

SECTION II. APPLIED BIOTECHNOLOGY IN THE PHARMACEUTICAL INDUSTRY... 387AccuracyThe analysis was performed on the CRM sample (Megazyme) containing a certified resistant starch content of 39.0 %u00b1 2.0%. Six repetitions were conducted, and the obtained values were compared with the published values to evaluate recovery. The analysis results are presented in Table 9.Table 9. Determination of Recovery on CRM SampleTrial m (mg) Abs Content (g/100g) CoA R%01 113.2 0.511 38.3639.0098.402 110.6 0.509 39.11 100.303 112.3 0.512 38.75 99.404 112.9 0.519 39.07 100.205 108.3 0.488 38.29 98.206 113.6 0.520 38.90 99.7Measurement UncertaintyThe evaluation results of accuracy and intermediate precision for the reference material (CRM) were used to estimate the measurement uncertainty, assuming that the error of the glucose standard solution is negligible. The results are shown in Table 10.Table 10. Measurement uncertainty of the methodSample R% Bias Bias^2 u(bias) u (Rw) u UCRM sample98.4 1.63 2.661,04 1,65 3,77 7,53100.3 -0.29 0.0899.4 0.65 0.42100.2 -0.17 0.0398.2 1.81 3.2799.7 0.25 0.06