Page 378 - Demo

382 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYTable 1. Results of the investigation of enzyme activity of hydrolyzing resistant starch%u03b1-amylase 30 CU/mL + amyloglucosidase RS content (%) Recovery0.3 U/mL 49.7 127%1.5 U/mL 42.6 109%3.0 U/mL 38.7 99.2%15 U/mL 33.0 84.6%Investigation of the hydrolysis time of digestible starchEach time point was repeated twice, and the average value was calculated. The recovery rate was determined by comparing the analyzed resistant starch content with the reference value. Table 2. Resistant Starch content in various hydrolysis timeTime RS content (%) Recovery2 h 45.1 116%4 h 44.5 114%6 h 43.2 111%8 h 42.8 110%12 h 39.2 101%14 h 39.1 100%16 h 38.9 100%20 h 39.0 100%24 h 37.5 96.1%30 h 34.6 88.7%36 h 31.4 80.5%Investigation of the correlation between recovery rate and the amount of sampleThe analysis was repeated three times at each sample weight, and the average value was calculated. The recovery rate was evaluated by comparing the analyzed resistant starch content with the published reference value.