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SECTION II. APPLIED BIOTECHNOLOGY IN THE PHARMACEUTICAL INDUSTRY... 379(Long An Province) %u2013 code sample: LA 1-4, and Quoc Oai District (Hanoi) %u2013 code sample: HN 1-2.- Four purple yam powder samples were collected from the Hanoi market: Code sample: KM 1-4.- Certified Reference Material (CRM) of resistant starch (Megazyme): 39.0% %u00b1 2.0%.2.2. Chemicals and ReagentsEnzymes and reagents provided by Megazyme: Amyloglucosidase (12 mL/vial, 3300 U/mL for starch substrate or 200 U/mL for p-nitrophenyl %u03b2-maltosidase substrate at pH 5.0 and 40%u00b0C. Pancreatic %u03b1-amylase (10 g/vial, 3 Ceralpha Units/mg). GOPOD reagent buffer solution (50 mL/vial, pH 7.4), p-hydroxybenzoic acid, and sodium azide (0.09% w/v). Enzyme for GOPOD reagent (lyophilized powder): containing Glucose oxidase, Peroxidase, and 4-aminoantipyrin. Glucose standard solution (5 mL/vial; 1.0 mg/mL) with 0.2% benzoic acid (w/v). Other chemicals and reagents (Maleic acid, Glacial acetic acid, NaOH, Sodium azide, Calcium chloride, KOH, Ethanol, etc.) of analytical purity.2.3. EquipmentsResearch using some analytical equipments: UV-VIS Biospectrometer Kinetic, measuring at a wavelength of 510 nm (BCE) with Quartz or plastic cuvettes, 10 mm thickness; Thermostatic shaking water bath (temperature 37 %u00b1 1%u00b0C; shaking speed 200 rpm/cycle) (Julabo); Analytical balance (Metler Toledo); Centrifuge (Hermle) ; vortex mixer, etc.2.4. Analytical method for RS contentAfter sample collection: fresh tubers were washed, peeled and crushed; powdered samples were mixed uniformly. Accurately