Page 381 - Demo

SECTION II. APPLIED BIOTECHNOLOGY IN THE PHARMACEUTICAL INDUSTRY... 385Table 6. Determination of LOD and LOQm (mg) V (mL) Abs Content (g/100g)Average (g/100g) SD0 LOD LOQ R105.2 10.3 0.205 1.711.69 0.06 0.18 0.60 9.35104.2 10.3 0.200 1.68107.9 10.3 0.212 1.72104.2 10.3 0.192 1.61108.4 10.3 0.209 1.69101.9 10.3 0.192 1.65109.2 10.3 0.221 1.77103.2 10.3 0.208 1.76105.1 10.3 0.190 1.58108.3 10.3 0.210 1.70The results show that the method has a limit of detection (LOD) of 0.18 g/100g and a limit of quantification (LOQ) of 0.60 g/100g. The R = Mean/LOD value falls within the 4 < R < 10 range, meeting the requirements. These values demonstrated that the method is applicable for analyzing resistant starch content in starchy food samples. RepeatabilityThe analysis was conducted on reference material samples (CRM-Megazyme), modified starch samples with high resistant starch content, cereal powder samples with medium resistant starch content, mung bean powder samples with low resistant starch content, and purple yam samples. Each sample was analyzed six times, and repeatability was assessed through the RSDr value. The results are shown in Table 7.