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SECTION II. APPLIED BIOTECHNOLOGY IN THE PHARMACEUTICAL INDUSTRY... 391The validation results showed that the method has a recovery range of 98.2% to 100.3%. According to AOAC guidelines, the recovery values should fall within the 98.0% - 101.0% range. When evaluating the bias from the CoA requirements, the obtained values ranged from 0.07% to 0.71%, all meeting the regulation of %u2264 2.0%. Therefore, the method for analyzing resistant starch content exhibited high accuracy and meets AOAC requirements. The results showed that the resistant starch content in fresh purple yam samples ranged from 10.4% to 16.7% (calculated as is); in purple yam powder samples, this value ranged from 10.6% to 13.2%. As a result, the resistant starch content in purple yam samples is relatively high. Our data indicated that purple yam (Dioscorea alata) has potential in the research and development of functional food and dietary supplement products to help control blood sugar and weight.5. CONCLUSIONSOur research applied enzyme hydrolysis techniques to analyze the resistant starch content in purple yam (Dioscorea alata). This plant is abundant in Vietnam and other tropical countries. The evaluation results indicated that this method is simple and easy to implement in food safety laboratories. Furthermore, method validation results demonstrated that the process is accurate and reliable. In conclusion, our findings can be applied in future studies to develop the raw materials in Vietnam as healthcare products for the public. However, the actual sample size collected was not large enough to accurately represent the resistant starch content in purple yam tubers in Vietnam. In the future, the study will continue to analyze other samples on the market to get clearer results.