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190 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYdiameters ranging from 26 - 30 mm; Strain N6 GII4 antagonized Bacillus cereus ATCC 11778 and Candida albicans ATCC 10231, with antagonism zone diameters ranging from 21 - 30 mm. Strain N1 AIA6 antagonized Escherichia coli ATCC 11105, with antagonism zone diameter of 17.3 %u00b1 0.6 mm, and simultaneously inhibited two experimental cancer cell lines, but did not inhibit HEK 293T normal cells. 16S rDNA sequencing results showed that strain N2 GII14 was 99.36% similar to Bacillus amyloloquefaciens, strain N6 GII4 was 100% similar to Bacillus siamensis, and strain N1 AIA6 was 99.78% similar to Acinetobacter sp.Keywords: Bacillus, Nai Lagoon, antibiotics, MCF-7 cells, Hep G2 cells 1. INTRODUCTIONMicroorganisms are a source of valuable bioactive substances with potential applications in many different industries, of which marine microorganisms are currently considered the most possible source [1].Coastal lagoons are transitional zones between land and sea with valuable ecosystems. They are one of the most valuable and diverse environments in the biosphere providing society with valuable ecological services, and high cultural heritage value. Lagoon environments occupy 13% of the world%u2019s coastal areas and are found along low-lying, tectonically stable coastlines. Due to their vulnerability, coastal lagoons, together with estuarine environments and coastal wetlands, have been identified as important transitional zones [2, 3]. Lagoon ecosystems have many distinct characteristics, are sensitive and vulnerable, and the environment constantly changes over space and time, leading to fluctuations in the species distributed in them. Therefore, the