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192 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYput in a sterile sample container, refrigerated and immediately transported to the laboratory for microbial isolation. The remaining sample was supplemented with 20% glycerol and stored at -30 oC. Figure 1. Sampling sites at Nai Lagoon (a- mangrove forest area in the lagoon, b- intersection area between the lagoon and rivers and streams, c- middle of the lagoon, d- 100m from the shore in the middle of the lagoon, e- lagoon mouth intersecting with Ninh Chu sea) (Source: Google map (left), and https://kienviet.net (right))Pathogenic bacterial used in the study: Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 6538, Staphylococcus epidermidisATCC 35984, Pseudomonas putida 3326K1, Pseudomonas auroginosaATCC 9027, Escherichia coli ATCC 11105, Salmonella typhimuriumATCC 14028, Enterobacter aerogenes ATCC 13048; Pathogenic yeast used in the study: Cadida albicans ATCC 10231, were provided by the VAST-Culture Collection of Microorganisms (VCCM), Institute of Biotechnology, Vietnam Academy of Science and Technology. Cell lines used in the study: MCF-7 breast cancer cells, and HEK