Page 189 - Demo

SECTION I: MOLECULAR GENETIC ENGINEERING AND BIOCHEMICAL TECHNOLOGY 193293T normal cells were provided by the Center for Research and Application of Stem Cells and Gene Therapy, Institute of Biotechnology; Hep G2 liver cancer cells were provided by the Institute of Marine Biochemistry, Vietnam Academy of Science and Technology.Several media were used to isolate bacteria from lagoon sediment samples. MA media contained NH4NO3 0.0016 g/L; H3BO3 0.022 g/L CaCl2 1.8 g/L Na2HPO4 0.008 g/L C6H5FeO7 0.1 g/L MgCl2 5.9 g/L MgSO4 3.24 g/L peptone 5.0 g/L KBr 0.08 g/L KCl 0.55 g/L NaHCO4 0.16 g/L NaCl 19.45 g/L NaF 0.0024 g/L Na2SiO3 0.004 g/L SrCl2 0.034 g/L yeast extract 1.0 g/L agar 15.0 g/L 1000 mL artificial seawater, pH 7.6 %u00b1 0.2 (25 oC). OLIGO media included Yeast extract 0.05 g/L trytone 0.5 g/L C3H7Na2O6P 0.1 g/L agar 15.0 g/L 1000 mL artificial seawater. SCA medium included Soluble starch 10.0 g/L K2HPO4 2.0 g/L KNO3 2.0 g/L casein 0.3 g/L MgSO4.7H2O 0.05 g/L CaCO3 0.02 g/L FeSO4.7H2O 0.01 g/L agar 15.0 g/L 1000 mL artificial seawater g/L pH 7.0 %u00b1 0.1. AIA media contained Sodium caseinate 2.0 g/L L-Asparagine 0.1 g/L sodium propionate 4.0 g/L K2HPO4 0.5 g/L MgSO4 0.1 g/L FeSO4 0.001 g/L agar 15.0 g/L pH 8.1 %u00b1 0.2 at 25 %u00b0C; artificial seawater 1000 mL. KA media contained Glycerol 10 mL; casein 0.3 g/L KNO3 2.0 g/L NaCl 2.0 g/L K2HPO4 2.0 g/L MgSO4.7H2O 0.05 g/L CaCO3 0.02 g/L FeSO4.7H2O 0.01 g/L agar 18.0 g/L artificial seawater 1000 mL. Gauze I media contained Soluble starch 20.0 g/L FeSO4.7H2O 0.01 g/L KNO3 1.0 g/L K2HPO4 0.5 g/L MgSO4.7H2O 0.5 g/L NaCl 2.0 g/L agar 15.0 g/L artificial seawater 1000 mL. Gauze II media contained Glucose 10.0 g/L NaCl 5.0 g/L peptone 5.0 g/L tryptone 3.0 g/L agar 15.0 g/L artificial seawater 1000 mL. MI media contained Soluble starch 10.0 g/L yeast extract 4.0 g/L peptone 2.0 g/L agar 18.0 g/L artificial seawater 1000 mL. Tested bacterial culture media (LB, g/L): peptone 10.0 g/L yeast extract 5.0 g/L NaCl 5.0 g/L distilled water 1000 mL g/L yeast culture media