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90 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYHydro-distillation of the fresh leaves gave a yellow oil with 0.16% yield (v/w). By the GC-FID/MS (gas chromatography-flame ionization detection/mass spectrometry) analysis, 26 compounds were identified, in which monoterpene hydrocarbons (62.2%) and their oxygenated derivatives (34.0%) were the main classes. The principal compounds included %u03b1-pinene (34.4%), myrtenyl acetate (20.7%), and %u03b2-pinene (17.3%). The obtained oil exhibited good activity in the antimicrobial assay. In particular, this oil with the MIC value of 16-128 %u00b5g/mL was better than streptomycin (MIC=256 %u00b5g/mL) in the antimicrobial assay against Gram (+) bacteria Staphylococcus aureus ATCC25923 and Enterococcus faecalis ATCC299212. By molecular docking study, the interactions between three major compounds, %u03b1-pinene, %u03b2-pinene, and myrtenyl acetate with S. aureus FtsA (PDB ID: 3WQU) and E. faecalis carbamate kinase (PDB ID: 2WE5) proteins showed that myrtenyl acetate displayed the best binding affinity with both two studied proteins and importantly interacted with residues in the active-site pocket. The in silico ADMET analysis revealed non-toxic property of the major compounds.Keywords: Siliquamomum oreodoxa; essential oil; antimicrobial; molecular docking; ADMET1. INTRODUCTIONSiliquamomum is a genus of flowering plants in the family Zingiberaceae [1-3]. Four species, including S. oreodoxa N.S.L%u00fd & %u0160korni%u010dk, S. alcicorne %u0160korni%u010dk. & H.%u0110.Tr%u1ea7n, S. phamhoangii L%u01b0u & H.%u0110.Tr%u1ea7n, and S. tonkinense Baill, were found to date, and all of them are distributed in Vietnam [1-3]. Among these species, S. tonkinense was the first species used in essential oil studies. Its leaf essential oil, which was collected from Nghean, was dominated by %u03b2-pinene (29.3%), %u03b1-pinene (15.7%), and sabinene (14.6%), whereas %u03b3-terpinene (14.9%) and o-cymene (14.0%) were