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SECTION I: MOLECULAR GENETIC ENGINEERING AND BIOCHEMICAL TECHNOLOGY 85methanol extract of Nigerian propolis successfully inhibited Cu. pipiens pipen fourth instar larvae with the LC50 values of 3.38 and 3.69 mg/L in tap and distilled water, respectively [16]. Table 3. Mosquito larvicidal activity of the isolated flavonoids 1 - 2No. LC50 (95% limits) LC90 (95% limits) %u03c72 pAe. aegypti (24-h) 1 39.62 (36.70-42.61) 59.76 (54.54-67.54) 1.2550 0.7402 59.69 (56.15-65.52) 76.22 (68.59-92.67) 0.0424 0.998Permethrin (control)0.0094 (0.0082-0.00107)0.0211 (0.0185-0.0249) 57.6 0.000Ae. aegypti (48-h)1 30.21 (28.37-33.23) 38.37 (34.54-46.48) 0.0333 0.9982 50.60 (47.51-53.96) 72.71 (66.05-84.81) 0.5866 0.899Ae. albopictus (24-h)1 39.85 (36.62-42.57) 51.74 (48.42-56.34) 0.0518 0.9972 54.48 (51.75-58.53) 71.82 (64.90-88.04) 4.0193 0.259Ae. albopictus (48-h)1 32.77 (30.31-36.41) 40.10 (36.15-47.39) 0.0033 1.0002 50.50 (46.83-52.19) 65.97 (60.47-78.63) 0.9038 0.8254. CONCLUSIONSThe current study briefly describes the isolation and structural determination of flavone santin (1) and flavanone sakuranetin (2) from the alcoholic extract of M. tenuiflora green propolis. Their strong DPPH free radical scavenging explained propolis%u2019s traditional and pharmacological uses for immunomodulatory targets. The values of these isolates also involved their inhibitory abilities against the Gram bacteria B. subtilis, S. aureus, E. coli, and P. aeruginosa, and the fungi A. niger and F. oxysporum, as well as Ae. aegypti and Ae. albopictus mosquito larvae. Further studies on in vivo molecular mechanisms of action are encouraged. Funding: This research was funded by S%u00e3o Paulo Research