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80 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYin our previous report [8]. In each assay, aliquots of essential oils diluted in ethanol (1% stock solution) were added to 250 mL beakers containing 150 mL of water and 20 fourth-instar mosquito larvae. Permethrin served as the positive control, while a separate set of controls consisting solely of ethanol functioned as the negative control. Mortality rates were recorded at 24 h and 48 h post-exposure, during which no dietary supplements were provided. The trials were conducted at a controlled temperature of 25 %u00b1 2 %u00b0C. Each experiment was replicated four times, utilizing five different concentrations (100, 50, 25, 12.5, and 6 %u00b5g/mL). The dead larvae were enumerated, and the average percentage mortality was subsequently calculated.In this analysis, M denotes the survival percentage in the control larvae population, while N represents the survival percentage in the treated larvae population. Log-probit analysis was conducted using Minitab%u00ae 19 (Minitab, LLC, State College, PA, USA) to evaluate the mortality data and to determine the lethal concentration values, specifically the LC50 (median lethal concentration) and LC90 (90% lethal concentration), along with their respective 95% confidence intervals. This statistical approach facilitates a robust estimation of the concentration at which the specified mortality percentages occur, aiding in the assessment of the efficacy of the tested compounds.3. RESULTS AND DISCUSSION3.1. Phytochemical analysisBio-guided fractionation of the green propolis alcoholic extract can lead to the isolation of two flavonoids 1-2. By nuclear