Page 72 - Demo

76 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYsakuranetin (2). Flavonoids 1 - 2 showed strong antioxidative activity to scavenge DPPH radicals with the IC50 values of 33.23 %u00b1 0.03 and 38.41 %u00b1 0.05 %u00b5g/mL, respectively. With the same MIC value of 64 %u00b5g/mL, they strongly inhibited the fungus Aspergillus niger ATCC 9587, whereas compound 1 also inhibited the fungus Fusarium oxysporum ATCC 11739. Compound 1 exhibited strong larvicidal activity against fourth instar mosquito larvae of Aedes aegypti, and Ae. albopictus.Keywords: Flavonoids, green propolis, Mimosa tenuiflora, antioxidant, antimicrobial, mosquito larvicidal1. INTRODUCTIONHoney bees, Apis mellifera L., produce propolis from various plant resins to protect their hive against invaders and regulate the temperature of their colonies [1]. Depending on the diversity of the flora, propolis can be found in several forms throughout the world. Propolis, which has complicated chemical compositions, is thus obtained from the resin that bees collect. For example, phenolic acid esters and flavonoids were found in samples from China and Europe, but polyprenylated benzophenone compounds were linked to propolis from Venezuela [2]. Mimosa tenuiflora (Willd.) Poir. (syn. M. hostilis), locally named %u201cJurema Preta%u201d, is a xerophilous species, which belong to the genus Mimosa, and the family Fabeceae. It can be found in beekeeping zones of the Northeastern Brazilian Caatinga [3]. The Caatinga green propolis has been produced in green since bees cut fragments of the young buds of plant to combine with wax [3]. Ferreira 2017 reported an HPLC qualitative chromatographic analysis of Caatinga green propolis extracts [4,5]. To date, chromatographic separations of green propolis extracts are quietly limited. The current research is aimed to highlight the