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70 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYTable 3. %u03b1-glucosidase inhibitory activity of compounds from Z. zerumbet rhizomesCompoundsInhibition (I, %) IC50(%u00b5M) 250 %u00b5g/mL100 %u00b5g/mL 50 %u00b5g/mL 25 %u00b5g/mL 10 %u00b5g/mLVanillic acid (1) 96.8 %u00b1 1.348.7 %u00b1 1.038.50 %u00b1 0.6025.60 %u00b1 0.45 14.80 %u00b1 0.99 102.8Naringenin (2) * 87.28 %u00b1 0.469.83 %u00b1 0.9158.97 %u00b1 0.68 42.97 %u00b1 0.86 19.6Acarbose 214.1*: I > 100 %; %u2013: I < 1 %According to the results, both compounds showed promising level of %u03b1-glucosidase inhibitory activity. The IC50 values of vanillic acid (1) and naringenin (2) are 102.8 and 19.6 %u00b5M, respectively, which are two-fold and eleven-fold lower than that of acarbose (IC50 214.1 %u00b5M). The results suggested that compound 1-2 are potential as antidiabetic agents for the inhibition of %u03b1-glucosidase enzyme.4. CONCLUSIONSTotal methanol residue, two extracts and two compounds from Z. zerumbet rhizomes have been investigated for their inhibition activity against %u03b1-glucosidase. As the results, chloroform extract showed good activity thus this extract was chosen for chemical investigation. As a result, two compounds, namely vanillic acid (1) and naringenin (2), were isolated. These compounds exhibited strong activity with significantly lower IC50 than that of the positive control (acarbose, 214.1 %u00b5M), particularly naringenin (2) with IC50 value of 19.6 %u00b5M. The potential %u03b1-glucosidase inhibitory activity of extract and compounds from Z. zerumbet rhizomes providing a theoretical basis for the application of these extract and compounds in functional foods, medicines or health products in supporting diabete treatment.