Page 45 - Demo

SECTION I: MOLECULAR GENETIC ENGINEERING AND BIOCHEMICAL TECHNOLOGY 49from Rhode island, USA in 1991 [16] and the second report was from Streptomyces sp. GEX1A isolated from the soil of Yamanashi prefecture, Japan in 2002 [17]. Six different analogs of herboxidiene have been reported from Streptomyces sp. GEX1A however only herboxidiene is known so far to be produced from S. chromofuscus ATCC 49982. To understand the range of analogs obtainable through the functional inactivation of reducing domains, it was essential to perform a basic profiling of herboxidiene analogs produced by S. chromofuscus. Thus, mass profiling of the natural analogs of herboxidiene was done through HR-QTOF mass analysis and the spectra related to corresponding mass [+Na+] of herboxidiene and 4 other analogs were found (Fig. 5). Each peak of PDA chromatogram obtained from S. chromofuscus culture extract were checked by HR-QTOF mass analysis. In Fig. 5, the peak at retention time of 5.50 showing the mass number of 461.2873 identified as herboxidiene as exact mass of herboxidiene [+Na+] is 461.2874. The peak at retention time of 5.07, 4.76 and 4.50 showing the mass number of 477.2823 identified as of GEX1Q1, GEX1Q2, and GEX1Q4 as exact mass [+Na+] of each of the compound is 477.2823. Finally, the peak at retention time of 5.42 shows the mass number of 447.2729 identified as an analog of GEX1Q5 with missing O-methyl functional group where exact mass of compound [+Na+] is 447.2717.