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SECTION I: MOLECULAR GENETIC ENGINEERING AND BIOCHEMICAL TECHNOLOGY 43Figure 2. Targeted modifications of herboxidiene PKS for generation various analogs2. MATERIALS AND METHODS 2.1. Bacterial strains, culture conditions, vectors and recombinant plasmidsAll the bacterial strains including wild type strains and the recombinants developed during this study are listed in Table 1. For cloning and sub cloning purposes, E. coli XL1-Blue MRF (Stratagene) was used whereas E. coli ET12567 (devoid of DNA methyl transferases) from John Innes Centre, UK, was used for demethylation of plasmids throughout the study. E. coli strains