Page 35 - Demo

ENGINEERING OF REDUCING DOMAINS OF HERBOXIDIENE PKS FOR GENERATING NOVEL ANALOGHue Thi Nguyen1,2+, Anaya Raj Pokhrel1+, Chung Thanh Nguyen3, Tuoi Thi Le1,4, Jay Kyung Sohng1,2,5,*AbstractHerboxidiene is a polyketide with a diverse range of activities, including herbicidal, anti-cholesterol, and pre-mRNA splicing inhibitory effects. Engineering of the polyketide synthase has been a productive tool for the generation of diverse analogs of various polyketides since the last 3 decades and few with enhanced or even new forms of activity as compared to parent molecules. To gain insights into the mechanism of tetrahydropyran (THP) ring formation in herboxidiene and 1 Department of Life Science and Biochemical Engineering, SunMoon University, 70 Sunmoon-ro 221, Tangjeongmyeon, Asan-si, Chungnam, 31460, Republic of Korea. 2 Anti-aging Bio Cell factory Regional Leading Research Center, Gyeongsang National University, Jinju, 52828, Republic of Korea.3 Institute of Biological and Food Technology, Hanoi Open University, B101 Nguyen Hien Street, Hai Ba Trung District, Ha Noi, Viet Nam4 Faculty of Biology, Hanoi National University of Education.5 Department of Pharmaceutical Engineering and Biotechnology, SunMoon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam, 31460, Republic of Korea. + The authors who have contributed equally to this work.* Corresponding authors: sohng@sunmoon.ac.kr