Page 47 - Demo

SECTION I: MOLECULAR GENETIC ENGINEERING AND BIOCHEMICAL TECHNOLOGY 513.4. Purification and analysis of the product of mutation strainUpon fermentation and analysis of the products from the mutant strain with desired module 8 DH domain inactivation, there was overall reduction in production of herboxidiene and its analogs. Results showed the 3 new spectra appeared on the chromatogram of mutant strain at retention time of 3.51 min, retention time of 4.59 min, retention time of 6.03 min (Fig. 6). Moreover, the overall metabolite production in the mutant strain is reduced as only limited number of PDA chromatogram appears upon HPLC analysis of S. chromofuscus %u0394M8DH mutant and the mutant strain suffers 1.3-fold reduction in herboxidiene production compared to wild type strain (Fig. 6). Figure 6. Comparison of PDA chromatograms of culture extracts from S. chromofuscus wild type and S. chromofuscus %u0394M8DH along with lambda max of major peaks (i) The chromatogram of S. chromofuscus wild type(ii) The chromatogram of S. chromofuscus %u0394M8DH