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48 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYPCR screening of the mutants was attempted but due to high homology of the modules and presence of DH domains in 5 among 8 modules of herboxidiene PKS, the amplified fragments were from other modules each of the time. Thus, screening of the mutant was performed based on the screening of the desired pattern of spectra on HPLC and confirmation by HR-Q-TOF mass analysis. Due to the co-linearity feature of polyketide synthase, the structure of the compounds can be tracked down based on the functional domains and number of modules present in PKS [15]. Figure 4. Confirmation of single crossing over by PCR of apramycin resistant gene from S. chromofuscus %u0394M8DH3.3. Identification of natural analogs of herboxidieneThe herboxidiene biosynthetic gene cluster has been reported so far from two of the strains. The first report was from S. chromofuscus ATCC 49982 (A7847) which had been isolated