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452 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYAcknowledgments: The authors would like to thank theScience and Technology project code TNMT.DL.2023.02 of the Ministry of Natural Resources and Environment for funding to carry out this research. Conflicts of Interest: The authors declare no conflict of interest.REFERENCES1. Alriksson B., Cavka A., J%u00f6nsson L.J. Improving the fermentability of enzymatic hydrolysates of lignocellulose through chemical in-situ detoxification with reducing agents, Bioresource Technology, 2011, 102, pp. 1254-1263.2. Kim T.H. Chapter 6. Pretreatment of lignocellulosis biomass. Bioprocessing Technologies in Biorefinery for Sustainable Production of Fuels, Chemicals, and Polymers., First Edition., Editor 1, Shang T.Y., Editor 2, Hesham A.E., Editor 3, Nuttha T., John Wiley & Sons, Inc.; Hoboken, NJ, USA, 2013, pp. 91%u2013110.3. Mu%u00f1oz S.S., Balbino T.R., de Oliveira F., Rocha T.M., Barbosa F.G., Mercado M.V., Marcelino P.R.F., Antunes F.A.F., Moraes E.J.C., dos Santos J.C., da Silva S.S. Surfactants, biosurfactants, and non-catalytic protein as key molecules to enhance enzymatic hydrolysis of lignocellulosis biomass, Molecules, 2022, 27 (23), p. 8180.4. Petridis L., Smith J.C. Molecular-level driving forces in lignocellulosic biomass deconstruction for bioenergy, Nature Reviews Chemistry, 2018, 2, pp. 382%u2013389.5. Chen Y.A., Zhou Y., Qin Y., Liu D., Zhao X. Evaluation of the action of Tween 20 non-ionic surfactant during enzymatic hydrolysis of lignocellulose: Pretreatment, hydrolysis conditions and lignin structure, Bioresour Technol. 2018, 269, pp. 329%u2013338.