Page 447 - Demo

SECTION II. APPLIED BIOTECHNOLOGY IN THE PHARMACEUTICAL INDUSTRY... 451In addition, studies also help to gain a deeper understanding of the mechanism of action of enzymes when being combined with additives, understanding the molecular dynamics of the process, and the influence of physicochemical factors (pH, temperature, and ionic strength) on the enzyme mechanism. It is also necessary to optimize experiments to screen variables and establish influence levels for selected variables to achieve the efficiency of enzymatic hydrolysis, saving costs for future production processes.5. CONCLUSIONSThe effects of Tween 20, Tween 80 and Triton X - 100 on the hydrolysis of cellulose in sugarcane bagasse by enzyme Cellic Ctec 2 in 48 hours of hydrolysis were evaluated, showing that the glucose concentrations accumulated were 92.5 g/L; 100.2 g/L; 87.5 g/L, respectively and the hydrolysis efficiencies were 41.1%; 44.5%; 38.9%.The effects of the hydrolysis conditions of cellulose in sugarcane bagasse with the participation of non-ionic surfactants were evaluated. When the hydrolyzed solution was carried out under optimal conditions at a solid residue concentration of 5%, pH 5, shaking speed of 150 rpm with the participation of surfactant Triton X - 100, the hydrolysis efficiencies were 62.5%; 70.0% and 75.1%, respectively.The hydrolysis of cellulose in sugarcane bagasse by enzyme combined with Triton X %u2013 100 took place in 60 hours under optimal conditions, showing that the glucose concentration reached 180.8 g/L, the hydrolysis efficiency was 80.4%. The results also showed that the addition of surfactants also significantly reduced the cost of enzymatic hydrolysis.