Page 328 - Demo

332 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGY3.2. The pradimicin A production of mutant strainsCrudes of A. hibisca wild-type and mutant strains were obtained from the culture broth of these strains for 7 days. The pradimicin A production of these strains was determined by HPCL (Table 1). Table 1. The pradimicin A production of A. hibisca wild-type and mutant strainsStrains Yeild of pradimicin A (mg/L) Increas (fold)A. hibisca P172-2 (wild-type strain) 8.6%u00b10.2 -A. hibisca M1 28.8%u00b10.1 3.3A. hibisca M2 38.0%u00b10.3 4.4A. hibisca M3 17.6%u00b10.3 2.0A. hibisca M4 52.4%u00b10.4 6.1A. hibisca M5 22.8%u00b10.5 2.7A. hibisca M6 12.7%u00b10.2 1.5A. hibisca M7 11.5%u00b10.3 1.3A. hibisca M8 24.6%u00b10.4 2.9A. hibisca M9 34.5%u00b10.3 4.0A. hibisca M10 11.5%u00b10.2 1.3A. hibisca M11 6.5%u00b10.3 0.8A. hibisca M12 37.5%u00b10.4 4.4A. hibisca M13 27.1%u00b10.3 3.2A. hibisca M14 48.6%u00b10.6 5.7A. hibisca M15 31.9%u00b10.4 3.7A. hibisca M16 38.4%u00b10.3 4.5A. hibisca M17 24.9%u00b10.5 2.9A. hibisca M18 29.3%u00b10.5 3.4A. hibisca M19 16.2%u00b10.3 1.9A. hibisca M20 36.7%u00b10.8 4.3Among the 20 mutant strains, 19 strains showed an increase in pradimicin A production compared to the wild-type strain, ranging from 1.3 to 6.1 fold, while 1 strain exhibited a decrease in pradimicin A production to the wild-type strain. Figure 1 shows the culture broth of A. hibisca M4, the mutant strain with the highest pradimicin A production, and A. hibisca P172-2,