Page 324 - Demo

328 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYa group known for its rich repertoire of bioactive secondary metabolites. A. hibisca P157-2 is known for its production of pradimicin A, a glycosylated polyketide. The structure of pradimicin A (Figure 1) is characterized by an aglycone composed of a dihydrobenzo[%u03b1]naphthacenequinone core, with a D-amino acid and a hexose sugar substitution [1,2]. The pradimicin A gene cluster comprises 28 open reading frames (ORFs), which encode a Type II polyketide synthase (PKS), enzymes involved in sugar biosynthesis and tailoring, as well as two resistance proteins. The predicted proteins exhibit significant similarities to those found in other angucyclic polyketide gene clusters, such as rubromycin, griseorhodin, and fredericamycin [3]. Pradimicin A exhibited good antifungal and antiviral activity [4]. The wild-type strain produces only 8.5 mg/L of pradimicin A. For enhancement of pradimicin A production, Sarita Paudel et al. was expressed regulator gene in A. hibisca P157-2. It led to increase the production of pradimicin A by 5.38 fold [5]. After that, two ABC transporter genes as pdmR1 and pdmR2 were overexpression in A. hibisca P157-2 resulting in a 5.4-fold increase in pradimicin A production compared to the wild-type strain [6]. However, it is still necessary to improve pradimicin A production.OH OOOOHOHOHOO NHOOHOOHOHNOOHOHOHChemical Formula: C40H44N2O18Exact Mass: 840.2589Figure 1. Structure of pradimicin A