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230 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYSome studies worldwide have shown that the chemical composition of avocado leaves contains major active compounds such as polyphenols and flavonoids, along with triterpene glycosides, coumarins, saponins, alkaloids, tannins, and reducing sugars [2]. Avocado leaves have been reported to possess several valuable biological activities, including: lipid-lowering effects [3,4], inhibition of Helicobacter pylori bacteria causing gastric ulcers [5], support for diabetes treatment [6], enhancement of memory [7], and antioxidant activity [8].However, according to the literature we have reviewed, there has been virtually no research on the chemical composition and biological activity of Vietnamese avocado leaves. In this article, we report the results of the isolation and structural identification of five compounds from avocado leaves harvested in Dak Nong province, Vietnam including luteolin (1), luteolin-7-O-glucoside (2), rutin (3), (6S,9R)-roseoside (4) and (7S,8R,8%u2019R)-5%u2019-methoxy isolariciresinol (5).2. MATERIALS AND METHODS 2.1. MaterialsAvocado leaves were harvested in Dak Nong province, Vietnam, in December 2023. The avocado leaves sample (Persea americana Mill.) was identified by Dr. Hung Quang Nguyen, Institute of Ecology and Biological Resources, VAST. The sample specimen number LB1223 is stored at the Institute of Natural Products Chemistry %u2013 Vietnam Academy of Science and Technology.2.2. Chemicals and EquipmentThin Layer Chromatography (TLC): TLC was performed on pre-coated plates DC-Aluofolien 60 F245 (Merck), RP-18 F254s (Merck). The spots were detected under ultraviolet light at two