Page 220 - Demo

224 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYintracellular reactive oxygen species (ROS) without causing evident cytotoxicity in the cells. Western blot analysis revealed that both pre- and post-fermentation products dramatically reduced iNOS and COX-2 protein expression in LPS-induced macrophages. Wu et al. (2013) found that pre- and post-fermentation products suppressed NF-%u03baB activation and LPS-induced TNF-%u03b1, IL-6, and IL-1%u03b2 production in a concentration-dependent manner [16]. The anti-inflammatory activity of L. paraplantarum MTCC 9483 strain was reported to be high during oxidative stress, LPSinduction, and pathogen invasion conditions, with reduced levels of IL-4, IL-10, and TLR-2 [17]. Study reported by Kim et al. 2019 on the metabolite profiles of mixed vegetables using L. plantarum strain found that fermentation increased antioxidative and/or anti-inflammatory agents like lactate, 3-phenylacetate, indole-3-lactate, %u03b2-hydroxybutyrate, %u03b3-aminobutyrate and glycerol [18]. Fermented MFP with L. plantarum and L. casei strains showed much higher anti-inflammatory activity in our study, with IC50values of 40.0 and 96.01 %u00b5g/mL, respectively. As a result, this study suggested the application of fermented MFP in skin care products or functional foods. 5. CONCLUSIONSThis study showed that the fermented MFP with L. plantarum, L. acidophilus, and L. casei strains had a significant increase in the density of beneficial bacteria. The increase in total phenolic content and antibacterial activity against S. aureus, MRSA, and E. coli, and especially the increase in anti-inflammatory activity of the fermented MFP, made the fermented MFP an ideal product for functional food or cosmetic ingredients.Conflicts of Interest: The authors declare no conflict of interest