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102 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGYThe reference compound, streptomycin, exhibits a low LD50value, indicating high toxicity. In general, the main components, %u03b1-pinene, %u03b2-pinene, and myrtenyl acetate, illustrate good compatibility as they comply with Lipinski%u2019s rule of 5, indicating their potential to exhibit drug-like characteristics through oral administration [17] and their low toxicity. Therefore, these compounds are promising candidates that require further research and development for future applications.For the residues Lys209 and Val206 of protein 2WE5, alkyltype interactions were established with %u03b1-pinene and myrtenyl acetate. %u03b2-pinene forms alkyl and pi-alkyl interactions with Pro270, Leu269, and Phe211 residues. Particularly, in the complex with protein 2WE5, a hydrogen bond was formed between the oxygen atom in the ether group of myrtenyl acetate and Asn51 important residue [14]. Overall, myrtenyl acetate exhibits the most promising potential contribution to antimicrobial activity against S. aureus and E. faecalis.Table 4. Physicochemical properties, the predicted LD50 value, and toxicity class of the major compounds in S. oreodoxa leaf essential oilCompound Molecular weight Log P HBD HBA%u03b1-Pinene 136 2.999 0 0%u03b2-Pinene 136 2.999 0 0Myrtenyl acetate 194 2.542 0 2Streptomycin 558 -2.068 16 18Compound MR Lipinski violationsLD50 (mg/kg)Toxicity class%u03b1-Pinene 43.752 0 3700 5%u03b2-Pinene 43.752 0 4700 5Myrtenyl acetate 54.711 0 2600 5Streptomycin 124.404 3 500 3