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298 PROCEEDINGS OF INTERNATIONAL SCIENTIFIC CONFERENCE ON APPLIED BIOTECHNOLOGY3.4. Effect of fermentation conditions(a) (b)(c) (d)Figure 3. Effect of different fermentation conditions as pH (a), temperature (b), moisture content (%) and incubation period (d) on pigment yield (O.D/g dry substrate) of the Monascus purpureus HG12 grown on rice branThe red pigment production of the tested fungal strain was found to be greatly influenced by the physical fermentation conditions used (Fig 3). The incubation temperature results (Fig 3b) showed that increasing the temperature increased the maximum pigment formation at 30 %u030aC, after which a decrease was observed at both levels (25-35 %u030aC).The data in Fig 3a show that, by increasing the pH value, the pigment production increased until reaching a maximum value